• USA Home
  • P9599 - Protease Inhibitor Cocktail

Sigma

Protease Inhibitor Cocktail

P9599 -

for plant cell and tissue extracts, DMSO solution

DOWNLOAD MSDS (PDF)

Description

Components

AEBSF
Bestatin
Pepstatin A
E-64
Leupeptin
1,10-Phenanthroline

Analysis Note

Tested for inhibition in extracts from Phaseolus vulgaris (kidney bean), Pisum sativum (pea), Triticum aestivum (wheat), Nicotiana tobaccum (tobacco), and Arabidopsis thaliana (arabidopsis).

Application

Tested on extracts from kidney bean, pea, wheat, tobacco, and Arabidopsis.

Other Notes

Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic, and metalloproteases, and aminopeptidases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), bestatin, pepstatinA, E-64, leupeptin, and 1,10-phenanthroline.

Quantity

One mL is recommended for the inhibition of proteases extracted from 30 g of plant tissue in a total volume of 100 ml.

Specificity

Inhibits serine, cysteine, aspartic, and matalloproteases, and aminopeptidases.

Physical form

Solution in DMSO

Price and Availability

Customers Also Purchased

for molecular biology, ≥98% (TLC), ≥99% (titration)

for use with fungal and yeast extracts, DMSO solution

for use with bacterial cell extracts, lyophilized powder


Documents

Certificate of Analysis

Certificate of Origin

P9599 - Datasheet (140 KB)
Plant Biotech Guide (395 KB )
Similar Products

Safety Information

WGK Germany1
Flash Point(F)185 °F
Flash Point(C)85 °C

related product

Product #

Description

P8340       Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
P8215       Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solution
P1860       Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution
P8465       Protease Inhibitor Cocktail, for use with bacterial cell extracts, lyophilized powder
P2714       Protease Inhibitor Cocktail, for general use, lyophilized powder
P8849       Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution
S8830       SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free, for use in purification of Histidine-tagged proteins
S8820       SIGMAFAST Protease Inhibitor Tablets, For General Use

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

References

Agne, B., et al., A Toc159 Import Receptor Mutant, Defective In Hydrolysis Of GTP, Supports Preprotein Import Into Chloroplasts. J. Thorac. Cardiovasc. Surg. 284, 8670-9, (2009)

Cole, M., et al., DORNROSCHEN Is A Direct Target Of The Auxin Response Factor MONOPTEROS In The Arabidopsis Embryo. Development 136, 1643-51, (2009) Abstract

Crozet, P., et al., Cross-phosphorylation Between Arabidopsis Thaliana Sucrose Nonfermenting 1-related Protein Kinase 1 (AtSnRK1) And Its Activating Kinase (AtSnAK) Determines Their Catalytic Activities. J. Thorac. Cardiovasc. Surg. 285, 12071-7, (2010)

Yin, L., et al., The Involvement Of Lipid Peroxide-derived Aldehydes In Aluminum Toxicity Of Tobacco Roots. Plant Mol. Biol. 152, 1406-17, (2010)

Liebminger, E., et al., 946;-N-Acetylhexosaminidases HEXO1 And HEXO3 Are Responsible For The Formation Of Paucimannosidic N-Glycans In Arabidopsis Thaliana J. Biol. Chem. 286, 10793-10802, (2011) Abstract

Wang, H., et al., Endogenous Protein Mono-ADP-ribosylation In Arabidopsis Thaliana. Planta 233, 1287-92, (2011) Abstract

Saathoff, A.J., et al., Downregulation Of Cinnamyl-alcohol Dehydrogenase In Switchgrass By RNA Silencing Results In Enhanced Glucose Release After Cellulase Treatment. PLoS ONE 6, e16416, (2011) Abstract

Yang, Y., et al., Regulation Of Flagellar Motility By The Conserved Flagellar Protein CG34110/Ccdc135/FAP50. Molecular Biology of the Cell 22, 976-87, (2011) Abstract


Share your experience with this product and how it worked in your specific application. We value your opinion and your colleagues will too!

Learn more at www.sigmaaldrich.com/reviews.

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?